Other

Part:BBa_K2082004:Design

Designed by: Mikail Sahin   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Monobody: Fusion protein of Monobody constant regions with RFP (exhange) and rpoZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
    Illegal AgeI site found at 1179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for E. coli


Source

Synthetic antibody mimetic.

References